Table 2 summarizes results of the. Picogreen Reagent b. 1 mL kit. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Figure 1. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Quantitation using Quant-iT Assay Kits. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. No. Quant Ittm Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. The assay is highly selective for double- stranded DNA over RNA, and in the. with the Qubit dsDNA BR Assay Kit and 1 µL of each sample was measured in quadruplicate with the Quant-iT™ PicoGreen dsDNA Assay Kit. Rapidly detect as little as 0. dsDNA만을 선택적으로 정량. 995 mL TE for the . assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Briefly, 2 µL of sample was diluted in 498 µL TE buffer. Regulations & Guidance; Manuals 0 0. Katalognummer: P11496. 99 to $1999. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Picogreen assays are compatible with most microplate readers and fluorometers. 微孔板读数仪. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. Quantitation Range. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Product Type. Mix by gently vortexing for 5 seconds. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. The Quant-iTTM dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. This sensitivity exceeds that achieved with the Hoechst 33258. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Q33130). Price from $9. • Specific for dsDNA in the presence of RNA. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Specific for dsDNA in the presence of RNA. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. This kit contains. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. Unit Size. Rapidly detect as little as 0. 2 to 100 ng) Assay Kit were used with a Qubit 3. 0). 12. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. PicoGreen Solution must be diluted fresh before each assay run. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 99. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Rapidly detect as little as 0. This protocol is a simplified and condensed. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. PicoGreen dsDNA quantitation reagents are. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Quant-iT PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. Catalog Number. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. 0 fluorometer and Quant-iT PicoGreen dsDNA. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 1 mL kit. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. Pico488 allows to quantitate as little as low pg/mL of dsDNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Prepare the assay. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. Picogreen assays are compatible with most microplate readers and fluorometers. 2. Similar. 8. Facebook. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. 3. ” application on Lunatic and compared to Qubit and. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 5mL. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. Rapidly detect as little as 0. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. 2. Product Type. Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. P11495. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. g. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. Begin typing to search, use arrow keys to navigate, Enter to select. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. 1 mL kit. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. Rapidly detect as little as 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen assay kit (Invitrogen, P7589) a. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. Showing 4 of 4. This protocol is a simplified and condensed version of. DNA standard curve. B–F are steps of the assay plate preparation using an test1. Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 2–400ng/µl (1µl sample in 200µl assay). quant it picogreen dsdna assay kit reagents. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. PicoGreen dsDNA quantitation reagents are. to each well. samples as well as standard in the assay itself. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 10 ng/mkl – 10000 ng/ml. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. Product Type. PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. PicoGreen assay. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). 50 ul PicoGreen Mix by swirling bottle. A comparison of reagents for detecting and quantitating proteins in solution—Table 9. no. Quant-iT PicoGreen dsDNA Assay Kit a. Nucleic acid quantitation using Quant-iT Reagents. 反应次数. 2–100 ng of DNA. , QuantIt PicoGreen dsDNA assay). Mit dem PicoGreen dsDNA. 86 / 100 stars. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. For ds DNA can be used kits below: Quant-iT™ PicoGreen ® dsDNA Assay Kit (Invitrogen P7589); Qubit® dsDNA Broad Range Assay Kit (Invitrogen Q32850);. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. For a standard. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. Rapidly detect as little as 0. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. Reagent Search & Data Services Menu Reagent Search. 99. 2 – 100 ng. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Suitable for use in 96- and 384-well plate formats. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. An aliquot (1. • Suitable for use in 96- and 384-well plate formats. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). 3 Q32854 26. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. Picogreen assays are compatible with most microplate readers and fluorometers. Quantitation Range. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. 2022. • Specific for dsDNA in the presence of RNA. Catalog Number. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 激发/发射. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. This was possible since, due to the low height of the light ray of only 8. 50 pg to 2 µg. 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. Quantitation Range. Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. Serial dilutions were made to give appropriate cell, seeding numbers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). PicoGreen dsDNA Assay Kit. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. 2023-11. 2009;49(4):997–1002. 作成者 LatB Staff 01. ru by: 1. Barcoded amplicons were pooled in. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Instead of 2 mL, only 1 mL measurement volume was used. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. in the assay in order to reduce waste and still achieve comparable results. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. • Specific for dsDNA in the presence of RNA. The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. • Specific for dsDNA in the presence of RNA. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. 50 pg to 2 µg. 製品番号（カタログ番号）： P7581. Picogreen assays are compatible with most microplate readers and fluorometers. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. • Suitable for use in 96- and 384-well plate formats. • Suitable for use in 96- and 384-well plate formats. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. • Suitable for use in 96- and 384-well plate formats. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. Catalog Number. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. One microgram of DNA was sheared using an M220. ITSkabir). • Suitable for use in 96- and 384-well plate formats. 326,933 327K. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. Quantitation Range. Control samples were not lysed and consisted of free pDNA. The PicoGreen reagent was discarded and the cells were rinsed with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) solution for 10 min. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. P11495. After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). 2. 2 to 100 ng) Assay Kit were used with a Qubit 3. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 10. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. For duplicate-well droplet. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). BR reagent (Component A) 1. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. Unit Size. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. PicoGreen dsDNA quantitation reagents are. 2. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 2. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Suitable for use in 96- and 384-well plate formats. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. 95 mL TE for the . • Suitable for use in 96- and 384-well plate formats. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. Qubit™ dsDNA Quantification Assay Kits. Quant-iT PicoGreen dsDNA Assay Kit. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. 21 DiB) [22]. DNA standard curve. 5 µL of each extracted sample was used for Picogreen analysis. Abstract. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. 9 Q33230 9 500 28. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. No. Picogreen assays are compatible with most microplate readers and fluorometers. 522. Rapidly detect as little as 0. Revised: 10–June–2008 | MP 07581 Table 1. 9. P7589. 2 ng to 100 ng of DNA in the assay tube. 0 mL to 200 uL to fit a 96-well microplate format. 产品概述. • Specific for dsDNA in the presence of RNA. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Highly selective for dsDNA over ssDNA or RNA. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. Rapidly detect as little as 0. PicoGreen dsDNA quantitation reagents are. 50 pg to 2 µg. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. 0. P7589. 8 Measurement of growth factor content in. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Rapidly detect as little as 0. 50 pg to 2 µg. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. The fusion protein nsp7-nsp8 was generated by inserting a GSGSGS linker sequence. Picogreen assays are compatible with most microplate readers and fluorometers. USA) and quantity was measured using Qubit 2. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. PicoGreen dsDNA quantitation reagents are. For duplicate-well droplet. The PicoGreen is sticking to the sides of the cuvette or assay plate. Three kits covering a wide range of dsDNA concentrations. This protocol is a simplified and condensed version of. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Buy from Supplier. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Picogreen assays are compatible with most microplate readers and fluorometers. Product Type. 2. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. For Research Use. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. The assay is linear over three orders of magnitude and has little sequence.